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high throughput microarray  (Illumina Inc)


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    Structured Review

    Illumina Inc high throughput microarray
    High Throughput Microarray, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high-throughput+microarray/high+throughput/pm40598589-68-5-13
    Average 90 stars, based on 1 article reviews
    high throughput microarray - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: MiR-101-3p Promotes Tumor Cell Proliferation and Migration via the Wnt Signal Pathway in MNNG-Induced Esophageal Squamous Cell Carcinoma.
    Article Snippet: Toxics 2024, 12, 824 3 of 17 ESCC patients’ tumor tissues and paraneoplastic tissue specimens of three newly developed ESCCs among permanent residents in Wuwei City, Gansu Province were selected for gene microarray high-throughput miRNA detection (Illumina Array 1 × 90K microRNA microarray high-throughput detection).

    Article Title: MiR-101-3p Promotes Tumor Cell Proliferation and Migration via the Wnt Signal Pathway in MNNG-Induced Esophageal Squamous Cell Carcinoma
    Article Snippet: ESCC patients’ tumor tissues and paraneoplastic tissue specimens of three newly developed ESCCs among permanent residents in Wuwei City, Gansu Province were selected for gene microarray high-throughput miRNA detection (Illumina Array 1 × 90K microRNA microarray high-throughput detection).

    Article Title: Type-2 diabetes epigenetic biomarkers: present status and future directions for global and Indigenous health
    Article Snippet: , Discovery cohort: n = 1,074 with T2D (67.3% male), n = 1,590 without T2D (68.2% male) (age and sex matched) Replication cohort: n = 377 with T2D (63.9% male), n = 764 without T2D (68.3% male) (age and sex matched) Liver association: n = 175 with obesity, a total of 2,201 blood samples and 116 liver samples were available for assessment , Incidence discovery cohort: Male and female people of Indian Asian descent from the LOLIPOP study. (Age range not described, mean for participants with T2D 52.5 years with SD ± 10.2 years, mean for participants without T2D 49.9 years with SD ± 9.8 years) Replication cohort: Male and female people of European descent from the LOLIPOP and KORA studies. (Age range not described, LOLIPOP group means: participants with T2D 60.7 years with SD ± 8.7 years and participants without T2D 60.4 years with SD ± 9.7 years. KORA group means: participants with T2D 57.8 years with SD ± 8.9 years and participants without T2D 57.6 years with SD ± 8.9 years) Liver association: People of European descent , Discovery cohort: A nested incident case-control. Blood-samples collected at baseline. Study follow-up period was 8-years. DNA methylation measured by Illumina HumanMethylation 450 K microarray. 466,186 probes tested. Validation done by assessment of the top findings in the replication cohort, which is an incident case-control design with blood-samples collected at baseline. DNA methylation measured by pyrosequencing with LOLIPOP participants and 450 K microarray with KORA participants. Liver association: in paired blood and liver samples. DNA methylation measured by HumanMethylation 450 K microarray. , After replication screening 5 CpG sites associated with T2D (p < 0.05). For these sites relative risk scores for each 1% increase in methylation were: ABCG1 (cg06500161) 1.09 (95% CI 1 · 07–1·11; p = 1·3 × 10– 1 7), PHOSPHO1 (cg02650017) 0·94 (0·92–0·95; p = 4·2 × 10 –11 ), SOCS3 (cg18181703) 0·94 (0·92–0·96; p = 1·4 × 10–9), SREBF1 (cg11024682) 1·07 (1·04–1·09; p = 2·1× 10– 1 0) and TXNIP (cg19693031) 0·92 (0·90–0·94; p = 1·2 × 10– 1 7). A T2D combined results methylation score comparing the RR of quartile 1 and quartile 4 produced a score of 3.51 (95% CI 2.79-4.42; = 1·3 × 10– 2 6) Liver association study observed association on TXNIP (p = 0.02) and SOCS3 (p = 5.3 × 10 −5 ).

    Article Title: Preimplantation genetic testing for structural rearrangements by genome-wide SNP genotyping and haplotype analysis: a prospective multicenter clinical study
    Article Snippet: The WGA products and genomic DNA of carrier couples, and the carriers' relevant parent if available, were processed by high throughput SNP microarray (Human Karyomapping-12; Asian Screening Array-24 v1.0; Illumina, San Diego, CA, USA) according to the manufacturer's instructions, and then scanned using the iScan Bead Array Reader.

    High Throughput Screening Assay:

    Article Title: The interplay of genetics and fatty acid metabolism: exploring their impact on metabolic syndrome in Swedish men.
    Article Snippet: .. SNPs were genotyped using a high throughput microarray technology, captured by either the Illumina 2.5 M or Cardio-Metabo chip (combined dataset). ..

    Article Title: Preimplantation genetic testing for structural rearrangements by genome-wide SNP genotyping and haplotype analysis: a prospective multicenter clinical study.
    Article Snippet: Shuo Zhang, Yuan Gao, ,g,h,i,aa Xiaohong Wang, Qing Li, Jichun Tan, Bo Liang, Ming Gao, ,g,h,i,aa Junping Wu, Xiufeng Ling, Jiayin Liu, Xiaoming Teng, Hong Li, Yun Sun, Weidong Huang, Xianhong Tong, Caixia Lei, Hongchang Li, ,g,h,i Jun Wang, Shaoying Li, Xiaoyan Xu, Junqiang Zhang, Wei Wu, Shanshan Liang, Jian Ou, Qiongzhen Zhao, Rentao Jin, Yueping Zhang, Chenming Xu, Daru Lu, Junhao Yan, ,g,h,i Xiaoxi Sun, Kwong Wai Choy, Congjian Xu,a,b,c,w,∗ and Zi-Jiang Chen ,g,h,i,s,t,∗∗

    Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level
    Article Snippet: Staining approach , Color-based barcode , 106 Ramos B cells are resuspended in different concentrations of the cell proliferation tracer CytoTell blue , - , - , S, RBD , n/a , 12 COVID-19 patient samples , Color-based barcoded spike protein flow cytometric assay (BSFA) , n/a , Label and separate samples , Comparison of immune responses triggered by different variants of SARS-CoV-2 , . .. Primer-associated approach , Sequence-based barcodes , Two unique barcodes embedded in primers at the stage of RT , + (the left and the right barcodes) , + , S , n/a , Commercial pooled human saliva from healthy individuals with spiked-in synthetic viral RNA , INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high- throughput test]; Illumina MiSeq (PE 150bp) , FASTX_trimmer , Multiplex samples , Diagnostics (48 samples) , . .. CRISPR-associated approach , Sequence-based barcodes , Customized peptide libraries are designed to encode a unique 20 bp nucleic acid sequence used as the gRNA barcode , - , - , S , n/a , COVID-19 samples (convalescent, pre-vaccine and post-vaccine) , Cas9 display (CasPlay) system (GenePix 4300A microarray scanner); Illumina NextSeq 500 (single-end 150 bp) used to sequenced dCas9-fusion library , GenePix Pro 7; Cutadapt v2.5 ) and customized commend lines , Multiplex samples , Evaluation of vaccine-induced antibody reactivities from the SARS-CoV-2 proteome , .

    Microarray:

    Article Title: The interplay of genetics and fatty acid metabolism: exploring their impact on metabolic syndrome in Swedish men.
    Article Snippet: .. SNPs were genotyped using a high throughput microarray technology, captured by either the Illumina 2.5 M or Cardio-Metabo chip (combined dataset). ..

    Article Title: Preimplantation genetic testing for structural rearrangements by genome-wide SNP genotyping and haplotype analysis: a prospective multicenter clinical study.
    Article Snippet: Shuo Zhang, Yuan Gao, ,g,h,i,aa Xiaohong Wang, Qing Li, Jichun Tan, Bo Liang, Ming Gao, ,g,h,i,aa Junping Wu, Xiufeng Ling, Jiayin Liu, Xiaoming Teng, Hong Li, Yun Sun, Weidong Huang, Xianhong Tong, Caixia Lei, Hongchang Li, ,g,h,i Jun Wang, Shaoying Li, Xiaoyan Xu, Junqiang Zhang, Wei Wu, Shanshan Liang, Jian Ou, Qiongzhen Zhao, Rentao Jin, Yueping Zhang, Chenming Xu, Daru Lu, Junhao Yan, ,g,h,i Xiaoxi Sun, Kwong Wai Choy, Congjian Xu,a,b,c,w,∗ and Zi-Jiang Chen ,g,h,i,s,t,∗∗

    Article Title:
    Article Snippet: .. Dataset Platform Sequencing type TCGA-LUAD Illumina HiSeq2000 RNA sequencing platform Expression profiling by high throughput sequencing GSE72094 GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray Expression profiling by array GSE135222 GPL16791 Illumina HiSeq 2500 Expression profiling by high throughput sequencing GSE91061 GPL9052 Illumina Genome Analyzer Expression profiling by high throughput sequencing ..

    Whole Genome Amplification:

    Article Title: Preimplantation genetic testing for structural rearrangements by genome-wide SNP genotyping and haplotype analysis: a prospective multicenter clinical study.
    Article Snippet: Shuo Zhang, Yuan Gao, ,g,h,i,aa Xiaohong Wang, Qing Li, Jichun Tan, Bo Liang, Ming Gao, ,g,h,i,aa Junping Wu, Xiufeng Ling, Jiayin Liu, Xiaoming Teng, Hong Li, Yun Sun, Weidong Huang, Xianhong Tong, Caixia Lei, Hongchang Li, ,g,h,i Jun Wang, Shaoying Li, Xiaoyan Xu, Junqiang Zhang, Wei Wu, Shanshan Liang, Jian Ou, Qiongzhen Zhao, Rentao Jin, Yueping Zhang, Chenming Xu, Daru Lu, Junhao Yan, ,g,h,i Xiaoxi Sun, Kwong Wai Choy, Congjian Xu,a,b,c,w,∗ and Zi-Jiang Chen ,g,h,i,s,t,∗∗

    Sequencing:

    Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level
    Article Snippet: Staining approach , Color-based barcode , 106 Ramos B cells are resuspended in different concentrations of the cell proliferation tracer CytoTell blue , - , - , S, RBD , n/a , 12 COVID-19 patient samples , Color-based barcoded spike protein flow cytometric assay (BSFA) , n/a , Label and separate samples , Comparison of immune responses triggered by different variants of SARS-CoV-2 , . .. Primer-associated approach , Sequence-based barcodes , Two unique barcodes embedded in primers at the stage of RT , + (the left and the right barcodes) , + , S , n/a , Commercial pooled human saliva from healthy individuals with spiked-in synthetic viral RNA , INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high- throughput test]; Illumina MiSeq (PE 150bp) , FASTX_trimmer , Multiplex samples , Diagnostics (48 samples) , . .. CRISPR-associated approach , Sequence-based barcodes , Customized peptide libraries are designed to encode a unique 20 bp nucleic acid sequence used as the gRNA barcode , - , - , S , n/a , COVID-19 samples (convalescent, pre-vaccine and post-vaccine) , Cas9 display (CasPlay) system (GenePix 4300A microarray scanner); Illumina NextSeq 500 (single-end 150 bp) used to sequenced dCas9-fusion library , GenePix Pro 7; Cutadapt v2.5 ) and customized commend lines , Multiplex samples , Evaluation of vaccine-induced antibody reactivities from the SARS-CoV-2 proteome , .

    Article Title:
    Article Snippet: .. Dataset Platform Sequencing type TCGA-LUAD Illumina HiSeq2000 RNA sequencing platform Expression profiling by high throughput sequencing GSE72094 GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray Expression profiling by array GSE135222 GPL16791 Illumina HiSeq 2500 Expression profiling by high throughput sequencing GSE91061 GPL9052 Illumina Genome Analyzer Expression profiling by high throughput sequencing ..

    Amplification:

    Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level
    Article Snippet: Staining approach , Color-based barcode , 106 Ramos B cells are resuspended in different concentrations of the cell proliferation tracer CytoTell blue , - , - , S, RBD , n/a , 12 COVID-19 patient samples , Color-based barcoded spike protein flow cytometric assay (BSFA) , n/a , Label and separate samples , Comparison of immune responses triggered by different variants of SARS-CoV-2 , . .. Primer-associated approach , Sequence-based barcodes , Two unique barcodes embedded in primers at the stage of RT , + (the left and the right barcodes) , + , S , n/a , Commercial pooled human saliva from healthy individuals with spiked-in synthetic viral RNA , INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high- throughput test]; Illumina MiSeq (PE 150bp) , FASTX_trimmer , Multiplex samples , Diagnostics (48 samples) , . .. CRISPR-associated approach , Sequence-based barcodes , Customized peptide libraries are designed to encode a unique 20 bp nucleic acid sequence used as the gRNA barcode , - , - , S , n/a , COVID-19 samples (convalescent, pre-vaccine and post-vaccine) , Cas9 display (CasPlay) system (GenePix 4300A microarray scanner); Illumina NextSeq 500 (single-end 150 bp) used to sequenced dCas9-fusion library , GenePix Pro 7; Cutadapt v2.5 ) and customized commend lines , Multiplex samples , Evaluation of vaccine-induced antibody reactivities from the SARS-CoV-2 proteome , .

    Multiplex Assay:

    Article Title: A systematic review of the barcoding strategy that contributes to COVID-19 diagnostics at a population level
    Article Snippet: Staining approach , Color-based barcode , 106 Ramos B cells are resuspended in different concentrations of the cell proliferation tracer CytoTell blue , - , - , S, RBD , n/a , 12 COVID-19 patient samples , Color-based barcoded spike protein flow cytometric assay (BSFA) , n/a , Label and separate samples , Comparison of immune responses triggered by different variants of SARS-CoV-2 , . .. Primer-associated approach , Sequence-based barcodes , Two unique barcodes embedded in primers at the stage of RT , + (the left and the right barcodes) , + , S , n/a , Commercial pooled human saliva from healthy individuals with spiked-in synthetic viral RNA , INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high- throughput test]; Illumina MiSeq (PE 150bp) , FASTX_trimmer , Multiplex samples , Diagnostics (48 samples) , . .. CRISPR-associated approach , Sequence-based barcodes , Customized peptide libraries are designed to encode a unique 20 bp nucleic acid sequence used as the gRNA barcode , - , - , S , n/a , COVID-19 samples (convalescent, pre-vaccine and post-vaccine) , Cas9 display (CasPlay) system (GenePix 4300A microarray scanner); Illumina NextSeq 500 (single-end 150 bp) used to sequenced dCas9-fusion library , GenePix Pro 7; Cutadapt v2.5 ) and customized commend lines , Multiplex samples , Evaluation of vaccine-induced antibody reactivities from the SARS-CoV-2 proteome , .

    RNA Sequencing:

    Article Title:
    Article Snippet: .. Dataset Platform Sequencing type TCGA-LUAD Illumina HiSeq2000 RNA sequencing platform Expression profiling by high throughput sequencing GSE72094 GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray Expression profiling by array GSE135222 GPL16791 Illumina HiSeq 2500 Expression profiling by high throughput sequencing GSE91061 GPL9052 Illumina Genome Analyzer Expression profiling by high throughput sequencing ..

    Expressing:

    Article Title:
    Article Snippet: .. Dataset Platform Sequencing type TCGA-LUAD Illumina HiSeq2000 RNA sequencing platform Expression profiling by high throughput sequencing GSE72094 GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray Expression profiling by array GSE135222 GPL16791 Illumina HiSeq 2500 Expression profiling by high throughput sequencing GSE91061 GPL9052 Illumina Genome Analyzer Expression profiling by high throughput sequencing ..

    Next-Generation Sequencing:

    Article Title:
    Article Snippet: .. Dataset Platform Sequencing type TCGA-LUAD Illumina HiSeq2000 RNA sequencing platform Expression profiling by high throughput sequencing GSE72094 GPL15048 Rosetta/Merck Human RSTA Custom Affymetrix 2.0 microarray Expression profiling by array GSE135222 GPL16791 Illumina HiSeq 2500 Expression profiling by high throughput sequencing GSE91061 GPL9052 Illumina Genome Analyzer Expression profiling by high throughput sequencing ..



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    Image Search Results


    Flowchart of the study Early-LUAD, early-stage lung adenocarcinoma; BLD, benign lung disease; NHC, normal healthy control; HuProt TM , Human Proteome Microarray; ELISA, enzyme-linked immunosorbent assay.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Flowchart of the study Early-LUAD, early-stage lung adenocarcinoma; BLD, benign lung disease; NHC, normal healthy control; HuProt TM , Human Proteome Microarray; ELISA, enzyme-linked immunosorbent assay.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Control, Microarray, Enzyme-linked Immunosorbent Assay

    Profiling of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control in the HuProt TM screening The heatmap displays a distinct distribution of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control, with generally higher levels of IgM observed in Early-LUAD. All values are normalized. The plots on the top display the data on age, sex, smoking, and alcohol consumption for each group, while the bar chart on the right side shows the sensitivity of each autoantibody. Control indicates the BLD+NHC group.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Profiling of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control in the HuProt TM screening The heatmap displays a distinct distribution of significant IgM autoantibodies between Early-LUAD and BLD/NHC/Control, with generally higher levels of IgM observed in Early-LUAD. All values are normalized. The plots on the top display the data on age, sex, smoking, and alcohol consumption for each group, while the bar chart on the right side shows the sensitivity of each autoantibody. Control indicates the BLD+NHC group.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Control

    Verification of autoantibodies by focused microarray A . Repeated detection of pooled samples displayed high reproducibility, with an averaged correlation coefficient of 0.95. Pooled samples comprised randomly selected samples from the Early-LUAD, BLD, and NHC groups (10 samples in each group). ***, P < 0.001 ( t -test for Pearson correlation coefficients). B . The top 10/top 15 IgG and IgM autoantibodies with the most significant elevation in Early-LUAD compared to BLD/NHC/Control are displayed. These most significant autoantibodies are ranked based on their FC on the vertical axis and sensitivity on the horizontal axis. C . A descending trend in the signal distribution of IgM autoantibodies across three representative samples from the Early-LUAD, BLD, and NHC groups, respectively. The images on the left visually depict the functionality of the focused microarray, while the 3D bar plots on the right show the distribution of normalized fluorescence intensities. D . IgG and IgM types of autoantibodies showed significantly higher levels in Early-LUAD compared to BLD. *, P < 0.05; **, P < 0.01 (Welch’s t -test). FC, fold change; NS, not significant.

    Journal: Genomics, Proteomics & Bioinformatics

    Article Title: A Novel IgG–IgM Autoantibody Panel Enhances Detection of Early-stage Lung Adenocarcinoma from Benign Nodules

    doi: 10.1093/gpbjnl/qzae085

    Figure Lengend Snippet: Verification of autoantibodies by focused microarray A . Repeated detection of pooled samples displayed high reproducibility, with an averaged correlation coefficient of 0.95. Pooled samples comprised randomly selected samples from the Early-LUAD, BLD, and NHC groups (10 samples in each group). ***, P < 0.001 ( t -test for Pearson correlation coefficients). B . The top 10/top 15 IgG and IgM autoantibodies with the most significant elevation in Early-LUAD compared to BLD/NHC/Control are displayed. These most significant autoantibodies are ranked based on their FC on the vertical axis and sensitivity on the horizontal axis. C . A descending trend in the signal distribution of IgM autoantibodies across three representative samples from the Early-LUAD, BLD, and NHC groups, respectively. The images on the left visually depict the functionality of the focused microarray, while the 3D bar plots on the right show the distribution of normalized fluorescence intensities. D . IgG and IgM types of autoantibodies showed significantly higher levels in Early-LUAD compared to BLD. *, P < 0.05; **, P < 0.01 (Welch’s t -test). FC, fold change; NS, not significant.

    Article Snippet: The high-throughput protein microarray HuProt TM v4.0 was obtained from CDI Laboratories (Catalog No. CDIHP-004, Baltimore, MD).

    Techniques: Microarray, Control, Fluorescence